Wednesday, February 20, 2013  |  Volume 5/Number 4  |  A Production of the BioProcessing Journal
Career Development
Partnering for Vaccine Emerging Markets
June 10-12, 2013
Berlin, Germany
Virginia Regional
Herpes Symposium
April 6, 2013
Norfolk, Virginia USA
Better Cells
for Better Health
June 23-26, 2013
Lille, France
April 16-18, 2013
Washington, DC Metro Area
3rd Annual Meeting
2 Tracks:
Application & Ancillary
March 11-15, 2013
Rosslyn, Virginia
Techno-Blast Masthead
Confocal micrograph: A549 cells
– Courtesy of Thomas Moninger, University of Iowa Central
Microscopy Research Facility
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to the editor today!
Suggested Reading
Improved Characterization of Bone Marrow Stromal Stem Cells
Could Support Their Use as Safe and Effective Therapies
By Samuel T. Mindaye, Moonjin Ra, Jessica Lo Surdo, Steven R. Bauer, and Michail A. Alterman
Products & Services
3D Digital PCR System
Life Technologies Corporation announces the QuantStudio™ 3D Digital PCR System, a scalable, chip-based instrument that is democratizing this technology for a rapidly growing market. Features include a simple workflow with minimal hands-on time and a chip-based platform to help reduce the risk of sample contamination as well as loss of DNA that is normally associated with droplet-based systems. The technology is ideal for accurately detecting rare mutations important in cancer or infectious diseases—the equivalent to finding a needle in a haystack. It is also increasingly used as a mechanism to perform quality control experiments on next generation sequencing libraries.

Designed with a scalable architecture, the QuantStudio 3D Digital PCR System’s first generation chips enable up to 20,000 data points in a single run—coverage that satisfies the needs for most digital PCR applications today. The capacity of future chips will increase exponentially to meet increasing demands of the research community.

For more information, please visit:
The new platform is the latest addition to a growing line of QuantStudio digital qPCR instruments from Life Technologies. It complements the QuantStudio 12K Flex Real-Time PCR System, which the company launched recently, and provides the highest throughput capability for digital PCR on the market.
Rapid Mycoplasma Detection Test Receives FDA Acceptance
Roche Diagnostics GmbH announced that the FDA has accepted the use of its MycoTOOL® PCR-based mycoplasma detection assay for release testing of one of Roche’s biological products. It is the first commercially-available mycoplasma PCR test accepted by the FDA for this application. MycoTOOL can replace conventional and time-consuming culture-based assay methods, reducing mycoplasma detection time from upwards of 28 days to one day.

Roche’s MycoTOOL PCR Mycoplasma Detection Kit incorporates all the necessary reagents for performing sample preparation and PCR reaction. It offers a high sensitivity (<1 CFU/mL for most isolates) and is applicable for many cell and cell-free sample types. In addition, this test detects the broad panel of Mollicute species as well, including over 150 species due to its universal primer design. Further, the MycoTOOL kit is reported to minimize the risk of false negative and false positive test results.

For more information, please visit:
Automated Workstation for Improved Protein Therapeutics Process Development
PerkinElmer has introduced the JANUS® BioTx Pro™ workstation designed to improve protein purification. The product is described as a highly flexible instrument that improves sample throughput enabling users earlier access to protein samples for further characterization. Designed for high throughput and small-scale protein purification, this workstation can accommodate multiple chromatography modes (column, tip, and batch) eliminating the need for multiple instruments.

By automating the purification function, this system enables scientists to obtain critical information earlier in the protein development pipeline. The JANUS BioTx Pro enables small-scale purification of µg to mg proteins on one system instead of three individual dedicated systems, supporting the use of commercially-available plate and column-based screening tools such as GE PreDictor™ plates, PhyNexus PhyTip® columns, and Atoll columns. Other applications for the workstation include resin binding studies and conditions screening.

For more information, please visit:
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  Career Development
BioDownstream Technologies Course 2013
April 15-19, 2013
Piran, Slovenia

2nd Workshop:
The New ParadIgM – IgM
from Bench to Clinic
April 23-24, 2013
Frankfurt, Germany
245th ACS National Meeting
& Exposition
April 7-11, 2013
New Orleans, Louisiana
BPJ Publishing