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Thursday, September 22, 2011  |  Volume 3/Number 12  |  A Production of the BioProcessing Journal
Career Development
New Virus Quantification Method Webinar
September 22, 2011
3:00 PM EDT
Whole Slide Image
Analysis & Stereology Seminars

September 23, 2011 Houston, Texas USA
September 26, 2011
Los Angeles, California USA
Protein Expression in Animal Cells Conference
September 25-29, 2011
Cascais, Portugal
Gene Therapy Symposium

September 26-27, 2011
Bethesda, Maryland USA
NCBI Discovery Workshop
September 27-28, 2011
Bethesda, Maryland USA
Mid-Atlantic Bio Symposium
October 12, 2011
Washington, DC USA
Microbial Fermentation: Development & Scale-Up
October 18-21, 2011
Logan, Utah USA
November 2-4, 2011
San Diego, California USA
FDA and Industry Perspectives on New Challenges and Innovation
November 8-10, 2011 Mayaguez, Puerto Rico
Suggested Reading
Gene Therapy with Viral Vectors
By Douglas J. Jolly, PhD, Tocagen
Defining a Detailed Approach to Using the Adenovirus Reference Material (ARM)
By Nancy Sajjadi and Janice Callahan, PhD, Sajjadi Consulting and Callahan Associates Inc.
Sponsored Links
New Bioreactor Adapter Kits Enable Single-Use Operation
Existing autoclavable bioreactors can now be adapted for use with New Brunswick Scientific’s 5 and 14 liter CelliGen BLU single-use vessels without the expense of replacing the entire controller. New Brunswick’s adapter kits enable a reduction in validation and utility requirements, as well as time and labor savings. Adapter kits for Applikon BioBundle controllers, as well as New Brunswick’s CelliGen 310 and CelliGen 115 systems, are currently offered while additional kits are in development.

CelliGen BLU single-use vessels feature a stirred-tank design for proven performance and scalability. Unique DO technology utilizing standard DO probes makes insertion completely noninvasive and reduces contamination risk. All vessel components that come in contact with product are made of USP Class VI materials and have been tested for leachables and extractables, making them appropriate for GMP environments. For more information, please visit www.nbsc.com/WBn.

New High Throughput Submarine Gel Electrophoresis Unit

Hoefer, Inc. introduces the new SUBHT High Throughput Submarine Gel Electrophoresis Unit to provide rapid, error-free nucleic acid analysis for a variety of applications. An entire 96-well plate can be quickly loaded in 8 easy steps with a multi-channel pipette, and the samples run in 30 minutes. Additionally, the fluorescent labeled, UV transparent gel running tray insures positive ID of loaded samples when placed on a UV transilluminator.

The SUBHT is easily optimized for a particular application with its large selection of combs and accessories. For more information, please visit www.HoeferInc.com.

Integromics Announces New Version of SeqSolve NGS Analysis Software

Integromics has released the second version of SeqSolve, its analytical solution for Next Generation Sequencing (NGS) data. This new version integrates state-of-the-art bioinformatics methods into one-click analysis workflows in an updated, more user-friendly graphical interface. Scientific visualizations are displayed with real-time interactive charts that can be exported into PowerPoint or as a PDF in one click.

Features include new applications for ChIP-seq and RNA-seq analyses including alternative splicing, significant improvements to the RNA-seq analysis, new gene discovery, and transcript-level differential expression. A new custom annotations loader extends the scope of the application to any species and proprietary genome annotations. Plus, SeqSolve’s smart quality control system produces consistent, biologically relevant, and reliable results by preventing experimental biases.

A client/server version has been implemented for large-scale scientific projects and will be publicly available in October. For more information, please visit www.integromics.com/ngs.
Techno-Blast Masthead
This Human Cervical Adenocarcinoma Cell (HeLa) culture was transfected with a chimeric enhanced cyan fluorescent protein (ECFP) plasmid vector that expresses a fluorescent fusion protein targeted at mitochondria. In addition, an enhanced yellow fluorescent protein (EYFP) plasmid vector was used that expresses a protein targeted at tubulin. The cells were also transfected with a recombinant plasmid vector containing a chimeric fusion gene product of DsRed2 fluorescent protein and a nucleus targeting sequence. Images were recorded in grayscale with a
12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles.

Image provided by Michael W. Davidson, National High Magnetic Field LaboratoryThe Florida State University, Tallahassee.

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Honors & Awards
One of ISBioTech's Founding Members Honored for His Work
on USU International Programs
  2nd Annual Meeting
  April 2-4, 2012
Rosslyn, Virginia USA

  Followed by the
1st Annual Meeting on:
 April 5-6, 2012
Rosslyn, Virginia USA
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