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Wednesday, August 24, 2011  |  Volume 3/Number 10  |  A Production of the BioProcessing Journal
Career Development
Introduction to
Electron Microscopy
for Life Sciences
September 7, 2011
11:00 am EDT
ISBioTech members receive 10% off the registration.
Preventing Costly
Out-of-Specification Investigations
Live Webcast
September 20, 2011
11:00 am EDT
Protein Expression in Animal Cells Conference
September 25-29, 2011
Cascais, Portugal
Gene Therapy Symposium

September 26-27, 2011
Bethesda, Maryland USA
NCBI Discovery Workshop
September 27-28, 2011
NIH Campus,
Bethesda, Maryland USA
Mid-Atlantic Bio Symposium
October 12, 2011
Carnegie Institution
for Science
Washington, DC USA
Control of Microbial Contamination in
Manufacture of Sterile
and Non-Sterile Products

Online Training Course
Suggested Reading
Report from the Lentivirus Vector Working Group:
Issues for Developing Assays and Reference Materials
for Detecting Replication-Competent Lentivirus in Production Lots of Lentivirus Vectors
Corresponding Author: Boro Dropulic, PhD, Lentigen Corporation
Electron Microscopy: Current Techniques Used in Product Safety
By Euan W. Milne, written while at Q-One Biotech Ltd. (acquired by BioReliance)
Sponsored Links
Cell Line Authentication Service

With more than two decades of experience in performing STR (short tandem repeat) on its extensive bio-repository, ATCC (American Type Culture Collection) now offers this service to the scientific community. Both the Basic (135-X) and Verified (135-XV) STR Profiling Services include a signed cell line authentication report containing an easy-to-understand STR allele table and electropherograms that support the allele calls at each locus.

The Verified Service also includes known reference profiling against the ATCC STR profile database, and a comprehensive interpretation of the results. Using ATCC's unique Sample Collection Kit with detailed instructions, researchers simply apply cells to the Whatman FTA card and ship their samples to ATCC (no need for dry ice). Investigators can expect emailed results within five days after arrival at ATCC.
For more information, please visit: www.atcc.org/STRprofiling.
New GENi2 Click and Capture Gel Documentation System
for Quick and Easy Imaging of any 1D DNA and Protein Gels

Syngene introduces its new GENi2 gel documentation system, the next generation of its GENi product line, to produce accurate images of fluorescent DNA and protein gels in seconds. The system features a compact darkroom containing a 2 megapixel resolution camera, motor-driven lens, and six-position motorized filter wheel, all controlled by an internal high speed processor. An integrated intuitive touch-screen allows quick set up for image capture, manipulation, and rotation.

The GENi2 darkroom also comes with a slide-out transilluminator and white light table. Interlocking doors automatically turn off the transilluminator when open, preventing UV exposure.

Images can be archived to a memory stick or local network connection and analyzed using GeneTools, Syngene’s image analysis software, which comes free of charge with the GENi2. For more information, please visit: www.syngene.com/geni2/.
New Flow Cytometer for Gentle Sorting of Large Libraries of 10-1,500 µ Particles

Union Biometrica's COPAS large particle flow cytometers are now available with a new high throughput screening (HTS) option package designed for applications involved in sorting large numbers of objects into vast numbers of microtiter output plates. COPAS models are built to handle bulky or fragile samples, including bead-based cellular or combi-chem libraries, large cells/clusters, small multicellular model organisms, and seeds.

The HTS option can be added to any new COPAS or BioSorter model and includes liquid level sensors, leak and clog detectors, as well as automatic sample pressure control for walk-away operation. A software option can also be added to pass system control to a robotic plate handler. For more information, please visit: www.unionbio.com.
Techno-Blast Masthead
Nuclear histone proteins were targeted in Madin-Darby Ovine Kidney Epithelial Cells (MDOK) with mouse anti-histone (pan) Mabs, which were imaged with goat anti-mouse Fabs fragments conjugated to Texas Red. The specimen was simultaneously labeled for the Golgi complex with Alexa Fluor 488 conjugated to goat secondary antibodies that target rabbit anti-giantin, and Alexa Fluor 350, conjugated to phalloidin, was utilized to counterstain the cytoskeletal F-actin network. Images were recorded in grayscale with a 12-bit digital camera coupled to either a Nikon E-600 or Eclipse 80i microscope equipped with bandpass emission fluorescence filter optical blocks. During the processing stage, individual image channels were pseudocolored with RGB values corresponding to each of the fluorophore emission spectral profiles. Image provided by Michael W. Davidson, National High Magnetic Field LaboratoryThe Florida State University, Tallahassee.

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  2nd Annual Meeting
  April 2-4, 2012
Rosslyn, Virginia USA

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  Followed by the
1st Annual Meeting on:
 April 5-6, 2012
Rosslyn, Virginia USA
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