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Forward
Techno-Blast
Friday, November 24, 2017  |  Volume 9 / Number 24  |  A Production of the BioProcessing Journal
ISBioTech 5th Fall Meeting
Career Development
Phacilitate
Cell & Gene Therapy World
&
Immuno-Oncology Frontiers World
January 23–25, 2018
Miami, Florida USA
BOKU
Protein Chromatography Course – Engineering Fundamentals and Measurement for Process Development and Scale-Up
May 27–June 1, 2018
Vienna, Austria
ACHEMA
ACHEMA
June 11–15, 2018
Frankfurt am Main, Germany
MSS 2018
8th Monolith Summer School and Symposium
June 15–20, 2018
Portorož, Slovenia
HPLC
HPLC 2018
July 29-August 2, 2018
Washington, DC USA
ISPPP
ISPPP 2018
November 4–7, 2018
Berlin, Germany
Suggested Reading
Opinion Editorial
By Donald L. Jarvis, PhD, Professor, University of Wyoming; and President, GlycoBac, LLC
Products & Services
New Clarification Platform Delivers Robust, Accelerated Performance Across Multiple mAb Processes
Stax mAx clarification platform

Pall Corporation introduces the Stax mAx clarification platform, a robust solution for the economic clarification of monoclonal antibody cell cultures without centrifugation or process additives. The single-use harvesting platform features a 4-layer concept, and has been optimized to maintain performance as cell density and viability change to minimize the impact of process variability between batches. Media configurations are available in many formats to scale directly from bench-top testing up to large-scale clinical production.

The pre-selected 4-layer, 2 stage platform features sequentially finer grades to remove aggregates, whole cells, and cell debris. Constructed to retain a small footprint across a wide range of feed streams (with cell densities of up 35 x 106 cells/mL, and feed turbidities of up to around 3,000 NTU), the Stax mAx clarification platform is scalable to support 1,000 to 2,000 L cell culture volumes. For more information, please visit pall.com.

Adaptive Focused Acoustics® Plate for Improved NGS Workflows

Covaris Inc. introduces the 96 oneTUBE-10 AFA Plate, which enables scalable and simplified workflows by leveraging Covaris' Adaptive Focused Acoustics® (AFA®) technology to meet rapidly increasing next-generation sequencing (NGS) demands for high-throughput and low-input library preparation. By using the oneTUBE-10 offering, transfer steps within the NGS library prep workflow have been eliminated from mechanical DNA shearing through end repair, A-tailing, ligation, and SPRI clean-up. The oneTUBE-10 product is specifically designed for use with Covaris' AFA technology for DNA shearing at volumes ranging from 10 to 50 µL, achieving highly accurate and reproducible results.

96 oneTUBE-10 AFA Plate

This new AFA vessel is made of engineered polymer for low impedance and efficient transmission of acoustic energy. oneTUBE-10 effectively controls acoustic cavitation to enable reproducible and precisely-tuned hydrodynamic shear forces. Designed to SLAS standards, oneTUBE-10 is compatible with standard heat blocks, magnets, and thermocyclers. oneTUBE-10 can be used on any liquid handling platform, providing a universal solution for streamlining NGS workflows. Paired with the LE220-plus (Covaris' newest focused-ultrasonicator), NGS workflows are easily standardized by integration with high-throughput laboratory automation.

Future applications of oneTUBE-10 include FFPE extraction, enhanced ligation enzymatic reactions, and other biomolecular processes. AFA can now be applied at various steps including bead binding, mixing, and elution, while scaling down to low volumes. For more information, please visit covaris.com.
New CRISPR Kits Simplify Gene Editing
CRISPR Complete Gene Editing Kits

Canopy Biosciences™, LLC has launched CRISPR Complete Gene Editing Kits. The CRISPR Complete Kits are the most comprehensive CRISPR kits available, including everything a researcher needs to perform gene editing with the CRISPR-Cas9 system.

The CRISPR-Cas9 system relies on two components—a targeting domain (sgRNA–orange) and a nuclease domain (Cas9–blue). This system allows for the generation of double-stranded breaks at highly specific and targeted sites within the genome. Kits are available for knockouts, knock-ins, point mutations, and many other project types, and include both design and synthesis of crRNAs, tracrRNA, Cas9 protein, genotyping primers, and donor (for knock-ins and point mutations). For more information, please visit canopybiosciences.com.

Career Development
CASSS
CMC Strategy Forum Japan
December 4–5, 2017
Tokyo, Japan
CMC Strategy Forum Winter
January 29, 2018
Washington, DC USA
WCBP
January 30–February 1, 2018
Washington, DC USA
AAPS
Annual Meeting & Exposition
November 12–15, 2017
San Diego, California USA
SLAS
SLAS International Conference & Exposition
February 3–7, 2018
San Diego, California USA
PREP
PREP 2018—31st International Symposium on Preparative and Process Chromatography
July 8–11, 2018
Baltimore, Maryland USA
ISBioTech 8th Spring Meeting
Advertising: Request a media kit!
Banner description: Human MyCell® Neurons can serve as a useful normal control for a wide variety of experiments. In this example, MyCell Neurons were co-stained for neuronal marker MAP2 (green) and nuclear marker MECP2 (red), for which loss-of-function mutations are associated with Rett Syndrome, a severe brain disorder that affects girls.
Courtesy of Cellular Dynamics International, a FUJIFILM company.
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