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Techno-Blast
Friday, June 9, 2017  |  Volume 9 / Number 12  |  A Production of the BioProcessing Journal
ISBioTech 5th Fall Meeting
Career Development
EVMS
8th Annual Virginia Viral Pathogenesis Symposium
June 24, 2017
Norfolk, Virginia USA
PREP
30th International Symposium on Preparative and Process Chromatography
July 16–19, 2017
Philadelphia, Pennsylvania USA
CASSS
CMC Strategy Forum Summer
July 17–18, 2017
Gaithersburg, Maryland USA
Mass Spectrometry
September 19–22, 2017
Boston, Massachusetts USA
ESGCT
XXV Anniversary Congress
October 17–20, 2017
Berlin, Germany
ISBioTech 8th Spring Meeting
Suggested Reading
Trends in Bioprocess Automation: Quality Control and
Process Analytical Technology in High Demand
By Kathleen Estes, PhD, and Eric S. Langer, MSB, BioPlan Associates, Inc.
Products & Services
New Bioprocess Control Station

Eppendorf introduces the BioFlo 120, their latest bench-scale fermentor/bioreactor system for research and development. This flexible instrument is capable of microbial fermentation as well as mammalian cell culture applications within a single platform. It features an extensive range of glass and BioBLU® Single-Use Vessel options (250 mL–40 L). Universal connections for digital Mettler Toledo® ISM and analog sensors make it easy to monitor a variety of critical process parameters.

The embedded software offers real-time local process control through an integrated touchscreen. The newly developed Auto Culture modes for push button control of microbial and cell culture applications drastically reduce the learning curve associated with new equipment. For additional process control capabilities and secure database management the BioFlo 120 can also be connected to Eppendorf SCADA platforms DASware® and BioCommand®.

For more information, please visit eppendorf.com.

BioFlo 120
RNA Kits for Streamlined NGS Library Preparation
NEBNext® Ultra™ II

New England Biolabs (NEB®) has expanded the NEBNext® Ultra™ II next-generation sequencing (NGS) library preparation product portfolio to include kits for RNA. The strand-specific NEBNext Ultra II Directional RNA Library Prep Kit and the non-directional NEBNext Ultra II RNA Library Prep Kit are both designed for Illumina NGS systems.

The kits are compatible with poly(A) mRNA isolation and ribosomal RNA (rRNA) depletion. Libraries can be constructed from a substantially broader input range (5 ng to 1 µg of total RNA) at high yields. Library complexity and transcript coverage are excellent even at low input amounts and with low quality samples.
The workflows include new reagents, combined steps, and minimized cleanup steps. NEB's protocols for constructing RNA libraries with Ultra II, including rRNA depletion or poly(A) mRNA enrichment, require under 30 minutes of hands-on time and can be finished in ~6 hours. The protocols are compatible with adaptors and primers from NEB or other sources. Both workflows are available in kit or module format, and with optional SPRIselect® beads for size-selection and cleanup steps.

For more information, please visit UltraIIRNA.com.

New Study Released on Cell-Ess Media

In a new bioproduction study by Essential Pharmaceuticals, LLC, Cell-Ess® media has been shown to increase antibody titer and improve glycosylation. Cell-Ess is a chemically-defined and animal component-free supplement and feed that delivers critical components, including essential fats, needed for cell growth. As a feed, Cell-Ess has been used in different CHO cell backbones and media formats to consistently improve protein titer by >20% compared to control. In the new study, Cell-Ess demonstrated an ability to improve glycan pattern consistency and complexity while also increasing titer.

With pressures to reduce the costs associated with bioprocessing, one way to address production costs is to optimize the efficiency of cell "factories" by increasing therapeutic protein yield. Cell-Ess consistently demonstrates the ability to increase antibody productivity when added as a feed or supplement to previously optimized systems. The increase was observed on a per cell basis, which means that downstream scientists do not have additional biomass to purify. Additionally, the increase is scalable to bioreactors, including single-use bioreactors. For more information, please visit EssentialPharma.com.
Cell-Ess®
Career Development
Intelligen
Workshop
Batch / Bio Process Modeling, Cost of Goods Analysis, Production Planning, Scheduling and Debottlenecking
June 2930, 2017
Boston, Massachusetts USA
PEACe
13th Conference on Protein Expression in Animal Cells
September 24–28, 2017
Valencia, Spain
BPJ Publishing
BioProcessing Journal Open Access
Techno-Blast Masthead
Induction of cell fusion by a newly described gene and its protein, called myomerger. Multi-nucleus fibroblast cells expressing genes needed to form skeletal muscle fuse together.

Courtesy of © Cincinnati Children's Hospital Medical Center
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