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Techno-Blast
Thursday, March 30, 2017  |  Volume 9 / Number 7  |  A Production of the BioProcessing Journal
ISBioTech 5th Fall Meeting
Career Development
International Serum Industry Association
12th Annual Meeting
May 3–4, 2017
Hong Kong
CASSS
Bioassays 2017:
Scientific Approaches & Regulatory Strategies
May 8–9, 2017
Silver Spring, Maryland USA
CMC Strategy Forum Europe 2017
May 22–24, 2017
Killarney, Ireland
ASGCT
20th Annual Meeting
May 10–13, 2017
Washington, DC USA
25th ESACT Meeting
25th ESACT Meeting
May 14–17, 2017
Lausanne, Switzerland
PREP
30th International Symposium on Preparative and Process Chromatography
July 16–19, 2017
Philadelphia, Pennsylvania USA
ISBioTech 8th Spring Meeting
Suggested Reading
Quality Risk Management (QRM): Part II — Evaluating the Impact of Process
Parameters on Critical Quality Attributes for Biopharmaceutical Products
By Mark F. Witcher, PhD, NNE
Products & Services
Novel Assay System to Accelerate Cell Line Development
Cy-Clone Plus assay system
IntelliCyt, a Sartorius Company, has launched the Cy-Clone™ Plus assay system for ranking and selection of clones for cell line development using the iQue™ Screener PLUS platform. A key factor in reducing the production costs of biopharmaceuticals is the development of cell lines producing a high yield of product. The Cy-Clone PLUS selection strategy is designed to identify clones that are highly productive and have optimal growth characteristics.
IntelliCyt’s iQue Screener PLUS platform is an integrated instrument, software, and reagent system that offers rapid, multiplexed analysis of cell and bead-based samples in 96 and 384-well plates. The iQue Screener’s proprietary sampling technology uniquely enables efficient assessment of cells in suspension, and miniaturizes the assay format, which conserves precious samples for further analysis. Profiling phenotypic and functional endpoints of cells, while simultaneously analyzing secreted proteins through the use of bead-based assays, generates rich information from the fewest numbers of cells. Plates are processed quickly, and software-assisted automation, analysis, and experiment-level visualization tools instantly rank and reveal the best clones for further analysis.

For more information, please visit intellicyt.com.
Next-Generation RNA Reprogramming and Stem Cell Services
Cellaria, LLC announces the expansion of its offerings to include a suite of stem cell services. The initiative advances Cellaria’s mission to provide more accurate patient and disease-specific cell models to help researchers focus and accelerate their research and discovery efforts.
Cellaria’s new stem cell division will provide RNA reprogramming and differentiation services in a comprehensive, yet modular workflow. The company’s initial reprogramming focus will be on custom solutions for primary cell establishment, induced pluripotent stem cell (iPSC) generation, banking, and characterization. Cellaria will also offer customizable differentiation services for various cell types such as neurons, hepatocytes, and skeletal muscle.
stem cell services
By incorporating the most advanced RNA technology, Cellaria’s reprogramming platform will enable access to a broad set of patient cells, including cells derived from human skin and blood, for the development of the next generation of disease models. With the addition of an extremely flexible and tailored differentiation system, Cellaria provides a single source solution to stem cell services.

For more information, please visit cellariabio.com/stemcell.
Human Genome-Wide Gene Expression Profiling Kit for 19,000 Human Genes
Cellecta, Inc. has launched the Driver-Map™ Human Genome-Wide Gene Expression Profiling kit, a targeted RNA expression profiling assay designed to easily provide a molecular snapshot of all known 19,000 human protein-coding genes from complex samples, starting from as little as 10 pg of total RNA.

Driver-Map™ Human Genome-Wide Gene Expression Profiling kit
The multiplex RT-PCR-based, followed by next-generation sequencing (NGS), method used in the kit allows access to specially designed and experimentally validated gene-specific primer pairs.

The Driver-Map Expression Profiling assay is highly reproducible. Noteworthy features of this assay include: selective amplification of 19,000 annotated human transcripts using RT-PCR directly from total RNA; an optimal genome-wide primer set that reliably and specifically measures mRNAs without background from other RNAs; and built-in, internal ERCC control RNAs to compensate for batch effects and other technical variations.
The kit includes a complete set of gene-specific and PCR-NGS primers, buffers, spike-in ERCC, and positive control RNAs, as well as all other reagents required to profile 24 samples and prepare them for digital expression profiling using NGS on an Illumina sequencing platform. NGS reagents are not included in the kit.

For more information, please visit driver-map.com.
Career Development
ISPPP
ISPPP 2017
July 19–21, 2017
Philadelphia, Pennsylvania USA
PEACe
13th Conference on Protein Expression in Animal Cells
September 24–28, 2017
Valencia, Spain
BPJ Publishing
BioProcessing Journal Open Access BioProcessing Journal BioProcessing Journal
Techno-Blast Masthead
Artistic rendering of mammalian cells.
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