Forward
Techno-Blast
Wednesday, March 1, 2017  |  Volume 9 / Number 5  |  A Production of the BioProcessing Journal
ISBioTech 7th Spring Meeting
Career Development
International Serum Industry Association
12th Annual Meeting
May 3–4, 2017
Hong Kong
CASSS
Bioassays 2017:
Scientific Approaches & Regulatory Strategies
May 8–9, 2017
Silver Spring, Maryland USA
ASGCT
20th Annual Meeting
May 10–13, 2017
Washington, DC USA
25th ESACT Meeting
25th ESACT Meeting
May 14–17, 2017
Lausanne, Switzerland
PREP
30th International Symposium on Preparative and Process Chromatography
July 16–19, 2017
Philadelphia, Pennsylvania USA
ISBioTech 5th Fall Meeting
Suggested Reading
A Novel Scalable Production Platform for Gene Therapy
Vectors Based on Human Suspension Cell Lines
By Kerstin Hein, Simon Fradin, Helmut Kewes, Martina Graßl,
and Nicole Faust, PhD, CEVEC Pharmaceuticals GmbH
Products & Services
First DNA Method to Authenticate Mouse Cell Lines
“A case of mistaken identity” is a tale of trouble for geneticists, oncologists, drug manufacturers, and others working with mouse cell lines. Cell lines that have been contaminated or misidentified due to poor laboratory technique and human error lead to inaccurate research studies, retracted publications, and wasted resources.
To help address this challenge, the National Institute of Standards and Technology (NIST) is working with partners to design tools, establish datasets, and further develop and standardize its system to authenticate mouse cell lines. NIST has been granted a patent for a mouse cell line authentication method using NIST-identified short tandem repeat (STR) markers that are non-coding. The technique is used to verify that a cell line is derived from a particular mouse. Once upcoming interlaboratory tests of the STR markers are complete, this will become the world’s first validated method for the authentication of mouse cell lines.
NIH 3T3 fibroblast cells
Additionally, NIST has partnered with ATCC to further develop the STR technology for authentication and establish the Mouse Cell Line Authentication Consortium. The consortium agrees to work with NIST and ATCC to test and validate the patented authentication method using the NIST-identified STR markers. It will also create a consensus standard to unify how authentication is performed across laboratories, and will establish a public database that defines which STR profiles identify which mouse cell lines. For more information, please visit nist.gov.
Quantitative RNA-Seq Service for Cost-Efficient and Accurate Gene Expression Profiling
RNA-Seq service
Lexogen now offers RNA-Seq service focusing on gene expression profiling using QuantSeq technology. Analysis of QuantSeq 3' mRNA-Seq Library Prep Kit data is based on counting of transcripts, making it straightforward and robust. The proprietary QuantSeq protocol focuses on capture of the 3' end of mRNAs, which is very tolerant of lower amounts and integrity of RNA input. Therefore, RNA extracted from clinical materials and formalin-fixed paraffin-embedded (FFPE) samples can also be submitted for accurate gene expression profiling.
The low read depth necessary for gene expression profiling by QuantSeq allows for a high degree of multiplexing, and automation of the library preparation procedure accommodates projects with higher sample throughput. The all-inclusive service costs 248 € per sample, and includes library preparation from submitted RNA, lane-mixing, sequencing, and all required quality controls for processing 48 samples concurrently. Data analysis down to differential gene expression is included in the report, with further analyses available upon request.

For more information, please visit lexogen.com.
Gene Expression Analysis Tool
ScienCell Research Laboratories, Inc. now supplies qPCR array kits and individual primers to study gene expression profiles. Primary cells are increasingly used by researchers as in vitro models of in vivo biological processes. ScienCell enables researchers to analyze gene expression variations using its customizable array kits and primers.
GeneQuery™ Human Schwann Cell Biology qPCR Array Kit
ScienCell's library contains thousands of validated primers for gene expression analysis, and their scientists can verify custom array kits and primers. One can choose a pre-designed array kit from 26 cell types and over 10 cell systems. The GeneQuery™ Human Schwann Cell Biology qPCR Array Kit is designed to facilitate gene expression profiling of 88 key genes involved in human Schwann cell biology such as peripheral nerve formation and regeneration, Schwann cell development and differentiation, myelination, and extracellular matrix (ECM) synthesis.

For more information, please visit sciencellonline.com.
Job Posting
GlycoBac
Research Scientist
Career Development
ISPPP
ISPPP 2017
July 19–21, 2017
Philadelphia, Pennsylvania USA
BPJ Publishing
BioProcessing Journal Open Access BioProcessing Journal BioProcessing Journal
Techno-Blast Masthead
Neuroblastoma cells throwing a Mardi Gras celebration.
Advertising
Economize by combining Techno-Blast and BioProcessing Journal advertising into one package.

Request a media kit today!
Did a colleague forward this to you? Subscribe here. While we'd hate to see you go, you can unsubscribe instantly.

[[tracking_beacon]]