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Techno-Blast
Thursday, April 14, 2016  |  Volume 8 / Number 8  |  A Production of the BioProcessing Journal
ISBioTech 4th Fall Meeting
Career Development
CASSS
CMC Strategy Forum
Europe 2016
May 9–11, 2016
Paris, France
ASGCT
19th Annual Meeting
May 4–7, 2016
Washington, DC USA
AAPS
AAPS National Biotechnology Conference
May 16–18, 2016
Boston, Massachusetts USA
PREP
29th International Symposium
July 17–20, 2016
Philadelphia, PA USA
HPLC 2016
44th International Symposium
June 19–24, 2016
San Francisco, CA USA
ISPE
Annual Meeting & Expo
September 18–21, 2016
Atlanta, Georgia USA
Cell and Gene Therapy
September 21–22, 2016
Berlin, Germany
Cell and Gene Therapy
ESGCT Annual Meeting
October 18–21, 2016
Florence, Italy
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Suggested Reading
Achieving Excellence in Biopharmaceutical Development and Manufacturing
by Using Appropriate Manufacturing Practices (AMPs)
By Mark F. Witcher, PhD, Integrated Project Services (IPS)
Sponsored Link
PATfinder™ – Lead The Way of Your Process
Production of high value biological therapeutics usually involves complex manufacturing processes with high process variability. PAT aims to enhance bioprocess understanding and implies a holistic approach to ensure that quality is built into products by design. Efficient PAT therefore calls for fast and robust analytical techniques which enables assessment of high quality information about critical quality attributes and key performance indicators as parallel as possible to the manufacturing process. PATfinder™ HPLC is the ideal system for routine gradient separations that enables you to do every analytical task. Equipped with bio-inert ceramic heads, it is deliberately tailored to meet the demands of analytical applications covering a wide range of biomolecules. Its highly sensitive and fast multiwavelength detector enables detection of component peaks even in very fast gradients.
User friendly HPLC system
HPLC fingerprinting approach
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PATfinder™ HPLC
BIA Separations
Products & Services
miRNA Select Assay Kits
Bioline, The PCR Company, a wholly-owned subsidiary of Meridian Bioscience, Inc., announces the worldwide release of the EPIK™ miRNA select assays, developed for the study of individual microRNA (miRNA) molecules. The EPIK miRNA select kits compliment the EPIK miRNA panel assays released toward the end of 2015.
EPIK™ miRNA select assays
These individual assays use the same novel algorithm approach developed by MiRXES™ for the panel assays, which employs miRNA-specific reverse transcriptase primers and hemi-nested real-time PCR primers along with Bioline reverse transcriptase, SensiSMART™ with SYBR®1 Green, and highly optimized buffers. The resulting assays enable detection of extremely low levels of miRNA with very high specificity, allowing the discrimination between closely related miRNA sequences.

For more information, please visit bioline.com.
Field-Flow Fractionation of Proteins, Macromolecules, and Nanoparticles
The Postnova AF2000 MultiFlow is a modular, high-performance flow fieldflow fractionation (FFF) platform that uses a unique crossflow field design. Samples are separated by their dynamic diffusion on the basis of molar mass or particle size. Because of this design and the absence of any stationary phase, FFF on the AF2000 MultiFlow can be performed without exerting shear forces and stress on the molecules or particles being separated.

The AF2000 can be run with different eluents under wide-ranging temperature conditions, and the same system can be used for running planar asymmetric flow FFF (AF4) or circular hollow fiber flow FFF (HF5) separations. Consequently, the AF2000 MultiFlow is an ideal platform for the characterization of proteins, antibodies, aggregates, vaccines, virus-like particles (VLPs), liposomes, nanoparticles, and natural or synthetic polymers.
AF2000 MultiFlow
Various add-on modules, extra options, and special FFF detectors are available, such as multi-angle light scattering (MALS) and dynamic light scattering (DLS). The AF2000 series has been designed for easy interfacing with high-end detection technologies from other suppliers, such as inductively coupled plasma mass spectrometry (ICP-MS), that may already be at use in your laboratory. For further information, please visit postnova.com.
Career Development
CIGB
Emerging Targeted Therapies in Cancer and Autoimmunity
April 21–25, 2016
Varadero, Cuba
BEBPA
Host Cell Protein Workshop
May 17–19, 2016
Lisbon, Portugal
ISIA
11th Annual Meeting
May 18–19, 2016
Barcelona, Spain
MSS2016
7th Monolith Summer School and Symposium
May 27–June 1, 2016
Portorož, Slovenia
BPJ Publishing
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Techno-Blast Masthead
iCell® Cardiomyocytes cultured at a low cell density, which can induce hypertrophic effects, for ten days and immunostained for sarcomeric alpha-actinin (clone EA53) in green and all-actin in red.

Courtesy of Cellular Dynamics International, a FUJIFILM company.
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