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Thursday, February 4, 2016  |  Volume 8 / Number 3  |  A Production of the BioProcessing Journal
Career Development
Phacilitate
7th Annual Virginia Regional Herpes Symposium
March 19, 2016
Norfolk, Virginia USA
CASSS
5th International Symposium on
Higher Order Structure of Protein Therapeutics
April 11–13, 2016
Long Beach, California USA
CMC Strategy Forum
Europe 2016
May 9–11, 2016
Paris, France
ASGCT
19th Annual Meeting
May 4–7, 2016
Washington, DC USA
BEBPA
Host Cell Protein Workshop
May 17–19, 2016
Lisbon, Portugal
PREP
29th International Symposium on Preparative and Process Chromatography
July 17–20, 2016
Philadelphia,
Pennsylvania USA
HPLC 2016
44th International Symposium on High Performance Liquid Phase Separations and Related Techniques
June 19–24, 2016
San Francisco, California USA
Suggested Reading
Selection of Clarification Methods for Improved Downstream Performance and Economics
By Sarah Le Merdy, Millipore S.A.S. France, a subsidiary of Merck KGaA
Products & Services
Rapid and Direct Quantification of Gene Therapy Viral Vectors

ViroCyt
has released the most recent addition to its ViroTag® portfolio: ViroTag AAV2-3. When combined with the Virus Counter® 3100, this reagent enables real-time quantification of adeno-associated virus serotypes 2 and 3, both of which are currently being developed as gene therapy vectors for a variety of different diseases.
ViroTag AAV2-3
Quantification of viral vectors is one of the most problematic steps during growth, harvest, purification and release. Traditional methods such as qPCR and absorbance readings at 260 nm and 280 nm are highly variable, resulting in over- or under-estimation of particles present at any given step. ViroTag enables direct, biologically-specific quantification of viruses in minutes by utilizing a fluorescently-labeled, high-affinity antibody targeted to the virus of interest. During a 1-minute sample analysis, the preparation is evaluated with the Virus Counter.

ViroTag AAV2-3 proves a valuable tool in process development, in-process testing, and release testing of AAV. For more information, please visit virocyt.com.
Real-Time Imaging of Cell Death
CAS-MAP™ CAS-MAP™ imaging reagents from Vergent Bioscience are cell-permeable imaging probes that identify apoptosis in whole cells, tissues samples, and live animals. The small molecular weight of the probes allow them to permeate the cell membrane and bind irreversibly to active caspases inside the cell where they will remain as long as the cell is intact. CAS-MAP imaging probes can be used with live animal imaging for real-time, non-invasive tracking of cell death. The non-toxic imaging probe uses the caspase family inhibitor VAD-FMK conjugated to FAM (FAM-VAD-FMK) as a fluorescent label. Unbound probe is washed (in vitro applications) or removed rapidly via circulation (in vivo applications). For more information, please visit vergentbio.com.
Routine Measurement of Protein Stability
Unchained Labs brings you the HUNK for complete automation of the chemical denaturation and protein stability measurement process.

Easy set up in application-specific trays is all it takes to get started. Once the HUNK gets going, it’ll crank out up to 96 ΔGs on 96 different conditions fully unattended. With those ΔGs in hand, you’ll know right away whether or not it’s worth your time to minimize aggregation or optimize solubility and viscosity.

The HUNK monitors the change in ΔG over increasing protein concentrations, and calculates the ΔG trend for you. Then HUNK tells you the fraction of denatured protein, the aggregated fraction, and how much of the denatured fraction is aggregated.

For more information, please visit unchainedlabs.com.
HUNK
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Career Development
CIGB
Emerging Targeted Therapies in Cancer and Autoimmunity
April 21–25, 2016
Varadero, Cuba
MSS2016
7th Monolith Summer School and Symposium
May 27–June 1, 2016
Portorož, Slovenia
BPJ Publishing
BioProcessing Journal BioProcessing Journal BioProcessing Journal
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