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Wednesday, October 15, 2014  |  Volume 6 / Number 21  |  A Production of the BioProcessing Journal
Career Development
On-Demand Webinar
Aseptic Management of the Sterility Testing Process
CMC Strategy Forum
Japan 2014
December 8–9, 2014
Tokyo, Japan
CMC Strategy Forum
and WCBP 2015
January 26–29, 2015
Washington, DC USA
Phacilitate Washington Bioleaders Forum 2015
January 26–28, 2015
Washington, DC USA
PDA Annual Meeting
March 16–18, 2015
Las Vegas, Nevada USA
Techno-Blast Masthead
An artistic illustration of mammalian cells dividing.

(Editor's note: I’ll take mine sunny-side-up with the DNA on the side.)

Email your cell images to the editor today!
Suggested Reading
From The Editor-in-Chief
By Keith L. Carson, BioProcessing Journal
Discuss on LinkedIn
Sponsored Link
Cell Counting Made Easy
Perfect for large to small scale applications, the Vi-CELL XR Cell Viability Analyzer automates the widely accepted trypan blue dye exclusion method for cell viability that has historically been performed manually with a light microscope, pipette, and a hemacytometer.

Thanks to cutting-edge liquid handling technology, the entire system — from sample aspiration to reagent handling to instrument cleaning — is fully controlled by an advanced, yet easy-to-use software interface designed for maximum flexibility.
To support a cGMP environment Beckman Coulter offers an Instrument Operational Qualification Program. This program is designed to reduce your time and effort spent on complying with equipment guidelines in GMP.

For more information, please visit:
http://www.particle.com/cell-counter/vi-cell-xr

Products & Services
New Mammalian Expression Platform
FUJIFILM Diosynth Biotechnologies has launched its new mammalian expression platform, named Apollo™. The platform is designed to create a robust ‘manufacturing-ready’ cell line and consists of: a new CHO DG44-derived host cell line; a novel expression vector; a cell line development process, utilizing state of the art screening technologies; and optimized media and feed components. This combination has been demonstrated to lead to a high producing recombinant cell line which can be used for pre-clinical material through to commercial production, and delivers titers up to 3 g/L before subsequent optimization.

In addition to high productivity, Apollo also offers robustness and speed to clinic for customers, and can be used throughout the entire product lifecycle. Using transfectant pools, the Apollo platform enables supply of representative material within 10 weeks of receiving a gene sequence, and a manufacturing-ready clonal cell line in 25 weeks from transfection. The platform has low regulatory risk and uses a host cell line with a proven track record.


For more information, please visit fujifilmdiosynth.com.
Efficient Quantification of Residual Protein A
Gyros AB announces its Gyrolab™ Protein A Kit for use with the Gyrolab xP workstation, a nanoliter-scale immunoassay platform.

During purification of biotherapeutics, Protein A can leach from the chromatography support and co-elute with the therapeutic antibody product. The contamination complicates subsequent analysis using immunoassays. In addition, Protein A can elicit an immune response. It is therefore essential to accurately quantify Protein A during the purification process, and prior to product release.
The new Protein A kit includes automated acid dissociation of Protein A and IgG during sample preparation to ensure contamination is accurately assessed. The kit also incorporates proprietary antibodies for high specificity and sensitivity; quantifies native Protein A, recombinant Protein A variants, and MabSelect™ SuRe ligand; and contains sufficient reagents to generate 240 data points. The sensitive, precise method provides high quality data within 80 minutes. Plus with fewer manual steps than other standard ELISA kits, the easy to use kit reduces hands-on time and risk for error.

For more information, please visit gyros.com.
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Career Development
Post Doc Position:
Bioprocess Technology
and Hybrid Modelling
Christian Doppler Laboratory, Vienna University of Technology, Austria
Position starts on
January 1, 2015
The 27th Annual Meeting of the Japanese Association for Animal Cell Technology (JAACT2014)
November 11–14, 2014
Fukuoka, Japan
The 12th Conference on Protein Expression
in Animal Cells
September 20–24, 2015
San Diego, California USA
BPJ Publishing






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