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Wednesday, April 2, 2014  |  Volume 6 / Number 7  |  A Production of the BioProcessing Journal
Career Development
Free Webinar
Fine Tuning Viral Clearance Approaches:
Enhancing Robust Steps & Defining the Limits
April 24, 2014
1:00 PM EDT
CMC Strategy Forum
Europe 2014
May 5–7, 2014
Sorrento, Italy
6th Monolith Summer School & Symposium
May 30–June 4, 2014
Portorož, Slovenia
Commercialization of
Gene and Cell Therapy Products Program
May 20, 2014
17th Annual Meeting
May 21–24, 2014
Washington, DC USA
Suggested Reading
A Working Definition of Quality by Design (QbD): Understanding its Application
to the Development and Manufacturing of Biopharmaceuticals
By Mark F. Witcher, PhD, Integrated Project Services (IPS)
We would like to encourage a discussion from you, our readers, on the topic of QbD.
Please email your feedback to the author or editor@bioprocessingjournal.com.
Products & Services
Reverse Signaling Apoptosis Bioassay
BioOutsource Ltd. has launched the flagship Reverse Signaling Apoptosis Bioassay, adding to the extensive range of characterization services offered by the company. The assay is a critical method required by regulators for licensure of Humira® (AbbVie) and Remicade® (Janssen) biosimilars.

The Reverse Signaling Apoptosis Bioassay replicates a critical component of TNF-alpha biology whereby membrane-bound TNF-alpha acts as a receptor, mediating a negative feedback system following binding by the TNF2 receptor. This results in cytokine suppression and the induction of apoptosis in TNF-alpha-producing cells, thereby dampening the elevated immune response.

For more information, please visit biooutsource.com.
Ultra Low Adhesion 3-D Spheroid Cell Culture


For more information,
please visit amsbio.com.
AMSBIO Lipidure®-COAT low adhesion plates and dishes are highly effective tools for state-of-the-art 3-D spheroid cell culture of stem cells (ES and iPS; human and mouse), NIH 3T3, pre-adipocytes, Hep G2 (and other cancer cell lines), as well as primary neuronal cells. These new plates and dishes are available in 384-well format, and are optimized for drug screening or other high-throughput applications.

Lipidure-COAT surfaces use MPC (2-methacryloyloxtethyl phosphorylcholine), a biocompatible synthetic polymer with the same structure as the phosphorylcholine polar bases that form cell membranes. Because of this, cells will not adhere to the surface of Lipidure-COAT plates or dishes, preventing the formation of satellite spheroids. Different well-shapes are available for 96-well plates (U, V, and flat-bottom) to control spheroid size, position, and density.
Secondary Antibodies for Fluorescent Western Blotting
Abcam has added Alexa Fluor® 680/790 secondary antibodies for fluorescent Western blotting to its Alexa Fluor conjugated antibody product line. Produced and validated extensively in Abcam laboratories, the F:P ratios of Alexa Fluor 680/790 have been optimized to ensure high quality and performance. The expanding range offers a broad portfolio of high-performing, labeled secondary antibodies with guaranteed bright staining and low background to meet the different needs of researchers undertaking multi-color analysis.


For more information,
please visit abcam.com.

The use of fluorescent Western blotting is growing and presents many advantages over the traditional method. With no need for film and chemiluminesence, fluorescent Western blotting is more convenient and enables greater signal stability with the ability to facilitate simultaneous multi-color analysis on one blot.
Techno-Blast Masthead
HeLa cells were transduced with CellLight® Mitochondria-GFP and Mitochondria-RFP reagents,
grown overnight, and imaged following counter-staining with Hoechst 33342.

Courtesy of Thermo Fisher Scientific.
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Career Development
PDA Annual Meeting
April 7–9, 2014
San Antonio, Texas USA
Partnering for Biologic Emerging Markets
May 28–29, 2014
Amsterdam, The Netherlands
5th Annual Virginia Regional Herpesvirus Symposium
April 12, 2014
Norfolk, Virginia USA
BPJ Publishing





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