Thursday, June 13, 2013  |  Volume 5 / Number 12  |  A Production of the BioProcessing Journal
Career Development
CMC Strategy Forum -
Summer 2013
July 15-16, 2013
Gaithersburg, MD USA
Setting Specifications for Biotechnology Products: Facing Evolving Challenges
September 25-26, 2013
Bethesda, MD USA

Suggested Reading
A Comparative Bioreactor Vessel Study: Conventional Reusable Glass
and Single-Use Disposables for the Production of Alkaline Phosphatase
By Taylor Hatton, Shaun Barnett, Ma Sha, PhD,
and Kamal Rashid, PhD, Center for Integrated BioSystems, Utah State University
Products & Services
New Innovative Genderless Sterile Connectors Simplify Systems Integration

View the spec sheet.
Click the Play button to download the demo. (10 MB)
For more information, visit colder.com.
Now available from Colder Products Company, the new Genderless AseptiQuik G Connectors enable quick and easy sterile connections, even in non-sterile environments. The connectors can be utilized for a wide range of applications including single-use bag and tubing assemblies, cell banking systems, bioreactors, mixers, sampling, and filling.

The innovative, easy-to-use genderless design simplifies system integration and minimizes the risk of operator error. The connectors' robust construction also delivers repeatable and reliable performance without the need for clamps, fixtures, or tube welders.

The G series is offered in both gamma and high-temperature versions, as well as a full range of interchangeable 1/4" to 3/4" flow solutions. Colder’s bioprocessing connections are manufactured in an ISO Class 7 certified cleanroom and meet USP Class VI material standards.
Fully-Integrated Mass-Directed Flash System
Biotage announces the new Isolera™ Dalton — a fully-automated, miniaturized mass-directed flash purification system. The system integrates, for the first time, a miniaturized mass detector and an advanced flash chromatography system into one commercially-available platform.

The mass detector and Isolera Spektra flash purification system are seamlessly integrated via the Isolera Dalton Nanolink unit, an intelligent sampling device which handles all fluids and synching calculations between the two units. Isolera Dalton easily fits inside a standard fume hood, requires no external pumps, and is capable of both normal and reverse-phase separations.

The instrument uses a wizard-based approach to method development, guiding users through the system with simple on-screen prompts. For more information, please visit biotage.com.
VersaComp Antibody Capture Bead Kit Provides Reagent Flexibility
VersaComp Antibody Capture Bead kits from Beckman Coulter Life Sciences are designed to set compensation for multicolor flow cytometry experiments by allowing utilization of the same reagents used in other experiments.
Two vials are provided. One contains positive beads — microspheres which capture conjugates in single-color stains. The second contains negative beads which provide particles with a measure of autofluorescence similar to unstained cells across the different excitation/emission wavelength combinations, including those excited by violet lasers. Using positive and negative beads for each conjugate allows generation of a compensation matrix to correct for the spectral overlap of that conjugate into other channels.

All mouse and rat isotypes, most hamster isotypes, and rabbit polyclonal IgG are recognized by the positive beads. The fluorescence generated by the positive beads after capture is well separated from the fluorescence generated by the negative beads.

The kits can be used with any cytometer including configurations with more than three lasers. For more information, please visit BeckmanCoulterReagents.com.
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Career Development
23rd ESACT Meeting:
Better Cells
for Better Health
June 23-26, 2013
Lille, France
Webinar:
Large Scale, CHO
Transient Transfection & Rapid Generation
of Stable Clones
June 19, 2013
11:00 AM EDT
26th International Symposium
July 14-17, 2013
Boston, MA USA
BPJ Publishing