Thursday, May 2, 2013  |  Volume 5 / Number 9  |  A Production of the BioProcessing Journal
Career Development
Best Practices in
Process Development
May 14, 2013
Durham, NC USA
26th International Symposium
July 14-17, 2013
Boston, MA USA
CMC Strategy Forum -
Summer 2013
July 15-16, 2013
Gaithersburg, MD USA
Microbial Fermentation:
Development & Scale-Up
May 21-24, 2013
Logan, UT USA
16th Annual Meeting
May 15-18, 2013
Salt Lake City, UT USA
Partnering for Vaccine Emerging Markets
June 10-12, 2013
Berlin, Germany
Download the PDF for $2.99
Outlook for Biotech
and Nanotechnology:
A New Generation
of Computer Chips
Suggested Reading
APPLICATION NOTE: Quantitative Endotoxin Assay of Ultrapure Water
By Katrin Toeppner and Dr. Elmar Herbig, Sartorius
Products & Services
Helium Integrity Testing
ATMI, Inc. announced that its proprietary helium integrity testing (HIT™) system has been made available to end-users for on-site applications. Originally introduced in 2011 as add-on testing for ATMI bioprocess vessels and manifolds, the HIT system is now also available in an easy-to-use technical transfer format that end-users can apply to standard and custom disposable bags and 3-D single-use assemblies starting from 300 L, with plans for application up to 1200 L.
The HIT system offers a more sensitive level of testing than standard pressure decay testing, and permits testing of the whole assembly rather than just the bag body. During the test, the single-use bag assembly or manifold is placed in a closed test chamber. Then a vacuum pump removes all of the air in the chamber, and a measured quantity of helium is injected into the assembly. Any helium leakage is measured by a mass spectrometer and the size of any defect is calculated. The test is capable of detecting defects as small as 10 µm.

ATMI also continues to offer integrity testing with the HIT system as an add-on test for ATMI vessels and systems.


For more information, please visit www.atmi.com, or email rbhella@atmi.com.
FLIPR Calcium 6 Assay Kits
Molecular Devices has launched FLIPR® Calcium 6 Assay Kits to address diversified GPCR and ion channel targets. The new kits feature a proprietary fluorophore dye that offers the highest quantum yield and delivers the greatest signal window available in a calcium assay kit. This substantial increase in signal enables monitoring of low-responders, including biorelevant reactions from endogenous, primary, or stem cell targets.
FLIPR 6 Calcium Assay Kits provide a comprehensive method for detecting intracellular calcium changes in a simple and reliable homogeneous assay format. Combining the novel fluorophore with proven proprietary quench technology, the new assay affords the highest signal to background ratio. The unique dye is more resistant to anion-exchange proteins, enabling measurement with minimal to no probenecid (an exchange inhibitor) when studying cell lines containing an organic anion transporter. Using a quench-free protocol, the FLIPR Calcium 6-QF Formulation allows assay performance without a masking technology, making it an ideal choice for quench-sensitive applications.

For more information, please visit www.moleculardevices.com.
Highly Specific Aptamer Protein Precipitation Kits
AMSBIO has introduced a new range of aptamer-based protein precipitation kits that bind with unmatched high specificity and affinity to target molecules. The new Aptoprecipitation (AP) / Co-aptoprecipitation (Co-AP) Kits are designed to provide easy, quick, and consistent protein precipitation.

Based upon a proprietary oligonucleotide, aptamer kits do not contribute to protein/peptide background that can interfere with subsequent mass analysis. The kits are already complete, supplied with aptamer covalently conjugated to magnetic beads, making successful pull down experiments easy to perform without the traditional troublesome conjugation step. The mild elution conditions used with aptamer kits enable isolation of intact proteins and protein complexes.
The AMSBIO aptamer (AP) resin is reusable and benefits from a long shelf life at room temperature, covalent magnetic bead immobilization, and mild elution methodology. The prepared aptamer-conjugated magnetic beads can also be regenerated and reused several times for AP / Co- AP assays without any significant loss in binding capacity.

For more information, please visit amsbio.com or email info@amsbio.com.
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Human dendritic cells (confocal micrograph).

Courtesy of Thomas Moninger, Assistant Manager, University of Iowa's Central Microscopy Research Facility, www.uiowa.edu/~cmrf/.
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Career Development
23rd ESACT Meeting:
Better Cells
for Better Health
June 23-26, 2013
Lille, France
Protein Characterization, Biopharmaceutical Development and Bioprocessing Workshop
June 4, 2013
Princeton, NJ USA
BPJ Publishing