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Thursday, May 31, 2012  |  Volume 4/Number 11  |  A Production of the BioProcessing Journal

   
Career Development
15th Planova
BioProcess Workshop

June 14-15, 2012
Cambridge, MA USA
Tutorials in Protein Purification: Hands-On, Intensive Lab Short Course
Flexible Dates in
June 2012
Email sujata.rutgers@gmail.com
for details.
Experiments with Green
Fluorescent Protein (GFP):
The Art and the Science
Multidisciplinary 3-Credit
Summer Lab Course for
Students from Grade School
Through Early College
June 25-August 3, 2012
Email crebb@rci.rutgers.edu
for details.
Both courses take place in:
New Brunswick, NJ USA
ASTM International Committee E48
on Biotechnology
June 27, 2012
San Francisco, CA USA

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Suggested Reading
Biopreservation of Cells for Use in Biotechnology and Cell Therapy
By Allison Hubel, PhD et al.,
Department of Mechanical Engineering, University of Minnesota
The Establishment of a Documentation System and Quality Control Strategy for the Plantibody HB-01 Production Employed in rHBsAg Purification for Pharmaceutical Use
By Lorely Milá Cáceres et al., Center for Genetic Engineering and Biotechnology
Sponsored Link

New Disposable Chromatography Housings from BIA Separations


These epoxy, thermoset-composite housings are carbon fiber-reinforced to provide the strength of stainless steel at a cost that makes them ideal for single-use or multi-use disposable applications. The product contact surfaces are coated with USP Class VI Parylene C which surpasses other polymer housings for chemical stability.

CIMmultus™ columns, available in 1 mL to 8000 mL volumes and pre-packed with CIM® monolithic resins, are the best choice for the purification or removal of viruses, plasmid DNA, protein aggregates, and macromolecules; as well as smaller molecules in a flow-through / polishing mode.


For more information, please go
to our website at CIMmultus™
advanced composite columns.
Products & Services

New Fill-It Operator Video Demonstration: Automated Cryovial Filling for Cell Banking

TAP Biosystems’ new demo includes step-by-step instructions on Fill-It key processes, including loading, priming, and calibrating the tube set, using function menus, processing the tubes, and cleaning the system. Each video shows how each task can be easily undertaken, as well as the efficiency and time-saving possibilities offered by Fill-It when compared to manual processing.

Fill-It is an automated system to rapidly dispense cell suspensions from bulk stock into cryovials, as well as decapping and recapping ready for freezing. Fill-It enables the rapid creation of high quality, consistent stock microtubes and cryovials for centralised cell line libraries, screening operations, or GMP production cell banks and can be used with both mammalian and microbial cell suspensions. The system may also be used for the rapid dispensing of other biological and non-biological liquids into screw top tubes.

For more information, please visit www.tapbiosystems.com.

CaptureSelect® C-tag Affinity Matrix for Highly Efficient Recombinant Protein Purification

BAC BV’s CaptureSelect C-tag affinity matrix combines exceptional selectivity for the shortest affinity tag available, E-P-E-A (glutamic acid-proline-glutamic acid-alanine), with a robust and high quality affinity resin. This allows one-step purification of any C-tagged protein with high purity and yield under both physiological as well as denaturing conditions. In addition, pH sensitive target proteins can be eluted under mild conditions ensuring high activity of purified protein.
The CaptureSelect C-tag affinity matrix is specifically designed to simplify workflow by reducing both time and cost whilst delivering higher quality output and increased flexibility in the protein purification process. At the C-terminal end of the protein of interest, only the EPEA sequence needs to be cloned. The CaptureSelect C-tag resin provides for highly specific purification of the tagged protein and is also suitable for use in high throughput processes as well as larger scale packed bed chromatography.

For more information, please visit www.captureselect.com/C-tag.
Techno-Blast Masthead
A culture of Madin-Darby canine kidney epithelial cells transfected with a pEYFP-Mitochondria chimeric plasmid subcellular localization vector. Cells were also stained with SYTOX Orange and Alexa Fluor 350 conjugated to phalloidin, targeting DNA and the cytoskeletal filamentous actin network, respectively.

Image provided by Michael W. Davidson, National High Magnetic Field Laboratory,
The Florida State University, Tallahassee.

Email your cell images to the editor today!
  Career Development
Drug Product for
Biological Medicines:
Novel Delivery Systems, Challenging Formulations and Combination Products
July 16-17, 2012
Bethesda, Maryland USA

How to Transform Cell Line Development and Increase Lab Productivity with ambr
Free Webinar
June 14, 2012
12:00 PM CET & 12:00 PM EST

Gene Expression &
Microarray Analysis

June 18-20, 2012
Downstream Processing of Biotechnology Products
September 11-14, 2012
Microbial Fermentation: Development & Scale-Up
October 16-19, 2012
All courses take place in:
Logan, Utah USA
BPJ Publishing
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