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Thursday, May 17, 2012  |  Volume 4/Number 10  |  A Production of the BioProcessing Journal

   
Career Development
Tutorials in Protein Purification: Hands-On, Intensive Lab Short Course
Flexible Dates in
May & June 2012
Email sujata.rutgers@gmail.com
for details.
Experiments with Green
Fluorescent Protein (GFP):
The Art and the Science
Multidisciplinary 3-Credit
Summer Lab Course for
Students from Grade School
Through Early College
June 25-August 3, 2012
Email crebb@rci.rutgers.edu
for details.
Both courses take place in:
New Brunswick, NJ USA
Animal and Human
Cell Culture:
Method and Applications

May 21-25, 2012
Bethesda, Maryland USA
Next Generation
Antibody Manufacturing
May 30-31, 2012
Boston, Massachusetts USA

Techno-Blast Masthead
Madin-Darby Canine Kidney Epithelial Cells (MDCK)

Image provided by
Michael W. Davidson,
National High Magnetic Field Laboratory, The Florida State University, Tallahassee.

Email your cell images
to the editor today!
Suggested Reading
An Investigative Comparison:
Plant-Based MAbs for Hep B Surface Antigen and MAbs from Mice
By Déborah Geada et al., Center for Genetic Engineering and Biotechnology
Personalized Cell-Based Medicine:
Activated and Expanded T Cells for Adoptive Immunotherapy
By Bruce L. Levine, PhD, The University of Pennsylvania School of Medicine
Sponsored Link
New Disposable Chromatography Housings from BIA Separations

These epoxy, thermoset-composite housings are carbon fiber-reinforced to provide the strength of stainless steel at a cost that makes them ideal for single-use or multi-use disposable applications. The product contact surfaces are coated with USP Class VI Parylene C which surpasses other polymer housings for chemical stability.

CIMmultus™ columns, available in 1 mL to 8000 mL volumes and pre-packed with CIM® monolithic resins, are the best choice for the purification or removal of viruses, plasmid DNA, protein aggregates, and macromolecules; as well as smaller molecules in a flow-through / polishing mode.


For more information, please go
to our website at CIMmultus™
advanced composite columns.
Products & Services
New Large Volume Transfection System
The AgilePulse® MAX from BTX is a new large volume transfection system ideal for fast, efficient transfection of 2 to 6 mL of cell suspension. Specifically engineered for large-volume applications, this system maximizes cellular uptake with minimal heating and short cycle-time to ensure high cell viability in further cell processing.

AgilePulse MAX technology offers direct scale-up from cuvettes to a large-volume chamber; ideal for gene or drug delivery, cancer immunotherapy, and protein therapeutics applications.

System features consist of patented Pulse Agile® technology, load meter for sample monitoring, and a 6 mL large volume sterile chamber. Additional benefits include direct scale-up from μL to mL volumes, and maximum efficiency with specialized cytoporation media.

Video tutorials for a variety of applications are available on the BTX website. For more information, please visit www.btxonline.com.
Quantitative Digital Pathology with Gene Probe Analysis
Definiens Tissue Studio® 3.5 now supports the automated analysis of in situ hybridization assays, including SISH, CISH, FISH, and dual-ISH. Tissue Studio facilitates the detection of spot-like signals originating from gene probes (or any other stain with spot-like appearance) on a cell-by-cell basis, and supports the detection of gene probe signals in both brightfield and immunofluorescene imaging.
Definiens Tissue Studio can be used to analyze images from any solid tissue with any stain, acquired by any scanner or microscope, and in any common file format. With just a single configuration, Tissue Studio automates the detection of regions of interest and has the capacity to distinguish individual cells and subcellular structures even in heterogeneous tissue samples.

The software provides quantitative readouts on morphological characteristics and molecular expression profiles of nuclear, cytoplasmic, and membrane biomarkers. In addition, Tissue Studio supports robust detection of vessels for automated angiogenesis assays as well as extensive multiplexing for the quantification of numerous parameters from the same tissue sample in immunofluorescence assays. Recordings of an introductory webinar series are available on Definiens’ YouTube channel.

For more information, please visit tissuestudio.definiens.com.
  Career Development
Drug Product for
Biological Medicines:
Novel Delivery Systems, Challenging Formulations and Combination Products
July 16-17, 2012
Bethesda, Maryland USA

Microbial Fermentation: Development & Scale-Up
May 22-25, 2012
Gene Expression &
Microarray Analysis

June 18-20, 2012
Downstream Processing of Biotechnology Products
September 11-14, 2012
All courses take place in:
Logan, Utah USA
BPJ Publishing
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